Glucose isomerase also called xylose isomerase (ECS. 3.1.5), of microbial enzymes, can will D a glucose, D a xylose, D-such as DNA into the corresponding ketone sugar, is one kind has significant economic benefit of YongMei industry. The in vitro certain conditions, because can catalytic D a glucose for D a fructose, so is the industry from its high starch large-scale fruit syrup of key enzymes.
This product conforms to the United Nations food and agriculture organization (FAO) and the world health organization (WTO), food and agriculture joint economic committee (UECFA) promulgated by the edible enzyme preparation standards. Industry, glucose isomerase is mass from starch preparation fructose syrup of key enzymes. Scientific research, glucose isomerase is the study of the enzyme and a catalytic mechanism protein engineering technology is one of the best model. This product is about 1.2 mm in diameter of brown irregular lever. The optimal pH7.5, the optimum reaction temperature 68 ℃. Glucose isomerase catalyzes D-glucose formation D-fructose isomerization reactions, is the industry to prepare high fruit syrup of key enzymes. Glucose isomerase is a heterogeneous immobilized enzymes. This paper proposes a new restructuring glucose isomerase (rGI) properties. RGI optimum effect temperature of 90 ° C ￣ 100 ° C, the optimal role for 7.0 pH. RGI has good thermal stability, 90 ° C of the enzyme live about 5 h half-life. RGI enzyme expression rely on live divalent metal ions. In the 90 ° C, pH7.0 and 5 h, rGI isomerization 30% (w/v) glucose liquid for fructose isomerization rate reached 54.8%.
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